Cell or tissue proteins were extracted following the protocol pro vided in the reagent kit
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Construction and screening of mutant strains
Cell growths were monitored every 8 h for reading at 600 nm using the nana-800 + ultra microspectrophotometer (Shanghai JP Analytical Instrument Co., Ltd., Shanghai, China).
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labeling Antibodies with (Ln3+)n-DNA Tags
a Nano-800 (Shanghai JP Analytical Instrument Co., Ltd.) spectrophotometer and stored at ?80 °C.
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a protein purifica-tion system
The harvested cells were resuspended in the lysis buffer con taining 50?mM Tris–HCl (pH 8.0), 100?mM NaCl, 1?mM CuCl2, 1?mg/L of lysozyme, and 10?µg/L of benzonase.